Part:BBa_K299105

J23100xbaJ61100

J61100 is an RBS and comes in a in plasmid pSB1A2, but there is also a J23100 promoter and XbaI site in the vector before J61100 RBS.

Biobrick 5' XbaI J23100 XbaI RBS Part Biobrick 3'

gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagaGAAAGAGGGGACAAactagtagcggccgctgcag

"Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely "T". The "G" downstream of the XbaI site obeys the standard. Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position."

The sequence of 61100 has been copied here together with the promoter sequence and the xba site to enable accurate in silico assembly of composite parts.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 37
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 37
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 37
  
• 1000
  COMPATIBLE WITH RFC[1000]